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Detection of human polyomavirus proteins, T‐antigen and agnoprotein, in human tumor tissue arrays
Author(s) -
Del Valle Luis,
Khalili Kamel
Publication year - 2010
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.21514
Subject(s) - virology , antigen , biology , microbiology and biotechnology , immunology
Abstract Expression of the human polyomavirus JCV genome in several experimental animals induces a variety of neural origin tumors. The viral proteins, T‐antigen and Agnoprotein, contribute to the oncogenesis of JCV by associating with several tumor suppressor proteins and dysregulating signaling pathways, which results in uncontrolled cell proliferation. In addition, T‐antigen and Agnoprotein have been associated with DNA damage and interfering with DNA repair mechanisms. In this study, we have utilized commercially available tissue arrays of human tumors of various origins and demonstrated the expression of both T‐antigen and Agnoprotein in some, but not all, tumors of neural and non‐neural origin. Most notably, more than 40% of human glioblastomas and greater than 30% of colon adenocarcinomas express viral proteins. The detection of viral transforming proteins, T‐antigen and Agnoprotein in the absence of viral capsid proteins suggests a role for JCV in the development and/or progression of human tumors. These results invite further large‐scale investigation on the role of polyomaviruses, particularly JCV in the pathogenesis of human cancer. J. Med. Virol. 82: 806–811, 2010. © 2010 Wiley‐Liss, Inc.