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Genetic diversity and zoonotic potential of human rotavirus strains, 2003–2006, hungary
Author(s) -
Bányai Krisztián,
Bogdán Ágnes,
Domonkos Gertrud,
Kisfali Péter,
Molnár Péter,
Tóth András,
Melegh Béla,
Martella Vito,
Gentsch Jon R.,
Szűcs György
Publication year - 2009
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.21375
Subject(s) - virology , rotavirus , genotyping , genotype , biology , phylogenetic tree , strain (injury) , genetic diversity , multiplex , virus , gene , genetics , medicine , population , environmental health , anatomy
Rotavirus strain surveillance is being conducted in many countries before and after introduction of newly licensed vaccines to assess the impact of the vaccines on rotavirus strains. Here we describe a strain surveillance study in the Budapest area of Hungary (2003–2006) based on RNA profile analysis, genotyping by multiplex PCR and nucleotide sequencing. Among 1,983 G‐typed rotaviruses we identified G1 (22%), G2 (4.8%), G3 (3.5%), G4 (18.5%), G6 (1.1%), G8 (<0.1%, n = 1), G9 (42%), and G12 (3.4%) specificities. Information on P genotype incidence was determined for a subset of samples (n = 814). In addition to the globally important strains, a variety of uncommon antigen combinations were also found, for example, P[9],G3; P[14],G6; or P[14],G8. Sequence and phylogenetic analysis of the VP7, VP4, VP6, and NSP4 genes of selected strains with uncommon antigen combinations demonstrated high similarity with certain bovine, porcine, feline, equine, and lapine rotaviruses, respectively. Continued surveillance is needed to assess the role of animal rotaviruses in human diseases. J. Med. Virol. 81:362–370, 2009. © 2008 Wiley‐Liss, Inc.