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Development and implementation of a molecular diagnostic platform for daily rapid detection of 15 respiratory viruses
Author(s) -
TiveljungLindell Annika,
RotzénÖstlund Maria,
Gupta Shawon,
Ullstrand Richard,
Grillner Lena,
ZweygbergWirgart Benita,
Allander Tobias
Publication year - 2009
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.21368
Subject(s) - virology , virus isolation , virus , immunofluorescence , isolation (microbiology) , respiratory tract infections , biology , respiratory system , respiratory tract , microbiology and biotechnology , immunology , antibody , anatomy
Acute respiratory tract infections are caused by a large number of viruses. Diagnostic methods have until recently been available only for a limited number of these viruses. With the objective to achieve sensitive assays for all respiratory viruses, a rational workflow in the laboratory, and a short turn‐around time, a real‐time PCR diagnostic platform for daily rapid detection of 15 respiratory viruses was developed. The system was evaluated on 585 stored nasopharyngeal aspirates from hospitalized children. Previous analysis by immunofluorescence and virus isolation identified viruses in 37% of the samples while the new PCR diagnostic panel detected 57% virus positive samples. The new platform was introduced in the laboratory in October 2007 and has then fully replaced the standard immunofluorescence assay for rapid detection of viruses and virus isolation. J. Med. Virol. 81:167–175, 2009. © 2008 Wiley‐Liss, Inc.