Premium
Molecular characterization of VP6‐encoding gene of group A human rotavirus samples from central west region of Brazil
Author(s) -
Tavares Talissa de Moraes,
de Brito Wilia Marta Elsner Diederichsen,
Fiaccadori Fabíola Souza,
Parente Juliana Alves,
da Costa Paulo Sérgio Sucasas,
Giugliano Loreny Gimenes,
Andreasi Márcia Sueli Assis,
Soares Célia Maria Almeida,
Cardoso Divina das Dôres de Paula
Publication year - 2008
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.21306
Subject(s) - rotavirus , biology , genotype , virology , capsid , gene , phylogenetic tree , genetic diversity , reoviridae , genome , polymerase chain reaction , genetics , virus , population , medicine , environmental health
Group A rotaviruses are the main cause of acute gastroenteritis in children worldwide. The intermediate capsid protein VP6 encoded by segment 6 of the dsRNA genome is the major structural component of the virus and it is highly antigenic and immunogenic. VP6 is responsible for group and subgroup (SG) specificities, allowing classification of group A rotavirus into SG I, SG II, SG I + II, and SG non‐I‐non‐II. VP6‐encoding gene of 154 group A human rotavirus samples of different G and P genotypes recovered from children in three cities of Central West region of Brazil was amplified by reverse transcription‐polymerase chain reaction followed by sequencing and phylogenetic analysis. Two distinct genetic groups could be recognized: VP6 genogroups I and II. Sequences analysis also revealed that all samples identified as VP6 genogroup I were associated with NSP4 genotype A, whereas samples identified as VP6 genogroup II were associated with NSP4 genotype B. This is the first study in Central West region regarding genetic variability of the VP6 gene. Further molecular surveillance of rotavirus strains is needed to understand better the occurrence of VP6 gene diversity in Brazil and the significance of VP6 for the control and prevention of rotavirus gastroenteritis. J. Med. Virol. 80:2034–2039, 2008. © 2008 Wiley‐Liss, Inc.