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A novel CD4‐conjugated ultraviolet light‐activated photocatalyst inactivates HIV‐1 and SIV efficiently
Author(s) -
Yamaguchi Koushi,
Sugiyama Takahiro,
Kato Shinji,
Kondo Yoichi,
Ageyama Naohide,
Kanekiyo Masaru,
Iwata Misao,
Koyanagi Yoshio,
Yamamoto Naoki,
Honda Mitsuo
Publication year - 2008
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.21235
Subject(s) - viremia , infectivity , virology , peripheral blood mononuclear cell , virus , biology , simian immunodeficiency virus , viral load , viral replication , microbiology and biotechnology , in vitro , biochemistry
In this study, we found that the electric potential derived from the redox reaction of ultraviolet (UV)‐illuminated CD4‐conjugated titanium dioxide (TiO 2 ) inactivated a wide range of high‐titered primary HIV‐1 isolates, regardless of virus co‐receptor usage or genetic clade. In vitro incubation of HIV‐1 isolates with CD4‐conjugated TiO 2 (CD4‐TiO 2 ) followed by UV illumination led to inhibition of viral infectivity in both H9 cells and peripheral blood mononuclear cells as well as to the complete inactivation of plasma virions from HIV‐1‐infected individuals. Treatment with a newly established extra‐corporeal circulation system with the photocatalyst in rhesus macaques completely inactivated plasma virus in the system and effectively reduced the infectious plasma viral load. Furthermore, plasma viremia and infectious viral loads were controlled following a second therapeutic photocatalyst treatment during primary SIV mac239 infection of macaques. Our findings suggest that this therapeutic immunophysical strategy may help control human immunodeficiency viral infection in vivo. J. Med. Virol. 80:1322–1331, 2008. © 2008 Wiley‐Liss, Inc.