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Virus load dynamics of individual CMV‐genotypes in lung transplant recipients with mixed‐genotype infections
Author(s) -
Görzer Irene,
Kerschner Heidrun,
Jaksch Peter,
Bauer Claudia,
Seebacher Gernot,
Klepetko Walter,
PuchhammerStöckl Elisabeth
Publication year - 2008
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.21225
Subject(s) - genotype , lung transplantation , immunology , biology , lung , bronchoalveolar lavage , virology , taqman , transplantation , cytomegalovirus , human cytomegalovirus , viral load , virus , medicine , viral disease , herpesviridae , real time polymerase chain reaction , genetics , gene
Human cytomegalovirus (CMV) is a major cause of disease and transplant dysfunction in lung transplant recipients. Simultaneous emergence of more than one CMV‐genotype can occur, and appears to be disadvantageous for the patient. In this study, the dynamics of individual CMV‐genotypes in blood and lung was assessed within mixed CMV‐genotype populations emerging after lung transplantation. In 69 plasma and 76 bronchoalveolar lavage samples of 16 lung transplant recipients with mixed CMV‐genotype infections within the first year posttransplantation each of the major glycoprotein B (gB) and glycoprotein H (gH) genotypes was selectively quantified by genotype‐specific quantitative TaqMan assays. The data obtained revealed that individually different genotype dynamics occurred for the individual patients and that the relative levels of the genotypes to each other may change over time. The quantitative development was independent of the specific gB–gH‐genotype. In 10 of the 16 lung recipients the patient's individual genotype composition was the same in blood and lung. Genotype development during the follow‐up was influenced by antiviral treatment. These data show for the first time that the CMV load used as diagnostic tool after transplantation is not always a constant entity but reflects the sum of the individual CMV‐genotype dynamics developing over time. J. Med. Virol. 80:1405–1414, 2008. © 2008 Wiley‐Liss, Inc.