Diagnosis of Oropouche virus infection by RT‐nested‐PCR | Zendy

Moreli Marcos Lázaro | Zendy; Aquino Victor Hugo | Zendy; Cruz Ana Cecília R. | Zendy; Figueiredo Luiz Tadeu M. | Zendy

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Diagnosis of Oropouche virus infection by RT‐nested‐PCR

Author(s) -

Moreli Marcos Lázaro,

Aquino Victor Hugo,

Cruz Ana Cecília R.,

Figueiredo Luiz Tadeu M.

Publication year - 2002

Publication title -

journal of medical virology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.782

H-Index - 121

eISSN - 1096-9071

pISSN - 0146-6615

DOI - 10.1002/jmv.2122

Subject(s) - virology , nested polymerase chain reaction , genome , biology , virus , polymerase chain reaction , genetics , gene

Using the RT‐PCR with primers that anneal to the 5′ and the 3′ extremities of the genome segments of bunyaviruses and internal primers that anneal to the S segment of Simbu serogroup viruses in a nested PCR it was possible to amplify the Oropouche virus (ORO) genome from the sera of three patients. These results show that this RT‐nested‐PCR is a useful tool for rapid diagnosis of Oropouche fever infections. J. Med. Virol. 66:139–142, 2002. © 2002 Wiley‐Liss, Inc.

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