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Diagnosis of Oropouche virus infection by RT‐nested‐PCR
Author(s) -
Moreli Marcos Lázaro,
Aquino Victor Hugo,
Cruz Ana Cecília R.,
Figueiredo Luiz Tadeu M.
Publication year - 2002
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.2122
Subject(s) - virology , nested polymerase chain reaction , genome , biology , virus , polymerase chain reaction , genetics , gene
Using the RT‐PCR with primers that anneal to the 5′ and the 3′ extremities of the genome segments of bunyaviruses and internal primers that anneal to the S segment of Simbu serogroup viruses in a nested PCR it was possible to amplify the Oropouche virus (ORO) genome from the sera of three patients. These results show that this RT‐nested‐PCR is a useful tool for rapid diagnosis of Oropouche fever infections. J. Med. Virol. 66:139–142, 2002. © 2002 Wiley‐Liss, Inc.