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Transport and budding at two distinct sites of visible nucleocapsids of West Nile (Sarafend) Virus
Author(s) -
Ng M.L.,
Tan S.H.,
Chu J.J.H.
Publication year - 2001
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.2101
Subject(s) - capsid , immunolabeling , immunoelectron microscopy , virology , flavivirus , biology , budding , viral replication , virus , vacuole , viral envelope , microbiology and biotechnology , cytoplasm , antibody , genetics , immunohistochemistry , immunology
It has been difficult to detect and visualize the physical nucleocapsid particles during the replication process of the flaviviruses. The use of cryo‐immunoelectron microscopy has clearly revealed the capsid proteins and nucleocapsid particles of West Nile (Sarafend) virus (a flavivirus) for the first time. Physical nucleocapsid particles accumulated in large numbers from 8 hr postinfection. Double immunolabeling of the envelope and capsid proteins showed a close association of these structural proteins for most of the replication cycle. By 10 hr postinfection, budding of nucelocapsids from the plasma membrane was very obvious. Although maturation at the plasma membrane was the dominant mode, during late infection, intracellular maturation into large vacuoles was also observed. J. Med. Virol. 65:758–764, 2001. © 2001 Wiley‐Liss, Inc.