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Anti‐norovirus polyclonal antibody and its potential for development of an antigen‐ELISA
Author(s) -
Okame Michio,
Shiota Tomoyuki,
Hansman Grant,
Takagi Makiko,
Yagyu Fumihiro,
Takanashi Sayaka,
Phan Tung Gia,
Shimizu Yuko,
Kohno Hideki,
Okitsu Shoko,
Ushijima Hiroshi
Publication year - 2007
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.20906
Subject(s) - polyclonal antibodies , virology , norovirus , antigen , genotype , capsid , antibody , monoclonal antibody , biology , recombinant dna , virus , epitope , cross reactivity , microbiology and biotechnology , gene , cross reactions , immunology , genetics
Abstract Norovirus (NoV) capsid proteins were expressed as virus‐like particles (VLPs) by using recombinant baculovirus in insect cells, which had 5 genotypes in genogroup I and 11 genotypes in genogroup II, and the VLPs were used as immunogens. Polyclonal antibody against the VLP of GII/3 genotype showed broad‐range cross‐reactivity, reacting not only with intra‐genogroup strains, but also inter‐genogroup strains, by antibody‐ELISA using 16 kinds of VLPs. Furthermore, antigen‐ELISA was conducted in sandwich enzyme‐linked immunosorbent assay (ELISA) using the polyclonal antibody for capturing antigens, and three kinds of monoclonal antibodies against the VLP of GII/4 genotype for detecting antigens. This format successfully detected eight genotypes of NoV from clinical specimens and proved that polyclonal antibody, which has broad‐range cross‐reactivity, was capable of detecting various types of genotypes from clinical specimens. J. Med. Virol. 79: 1180–1186, 2007. © 2007 Wiley‐Liss, Inc.