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Longitudinal study of serum neutralization of HIV‐1 in infected plasma donors
Author(s) -
Abelian Arthur V.,
Saurya Shreesh,
Ash Stephen,
Karpas Abraham
Publication year - 2001
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.2086
Subject(s) - plasmapheresis , virology , neutralization , syncytium , titer , immune system , immunotherapy , immunology , medicine , human immunodeficiency virus (hiv) , neutralizing antibody , polyclonal antibodies , antibody , antigen , cytopathic effect , virus
Earlier studies provided suggestive evidence about the effectiveness of passive immunotherapy for AIDS patients using plasma donated by healthy HIV‐1 infected individuals and revealed beneficial effects of plasmapheresis for the immune system of the donors. In this study, anti‐HIV‐1 neutralizing activity in 31 healthy HIV‐1 infected donors of plasma participating in a passive immunotherapy study was investigated as a function of time. Average studied period was 33 months. Using the highly cytopathic HIV‐1 NDK strain and MOLT4 cells, it was shown by means of syncytia formation inhibition assay and polyclonal HIV‐1 antigen‐capture assay that viral neutralizing titers tend either to remain unchanged or increase over time. These findings support the notion that the immune system is not affected adversely in HIV‐1 infected plasma donors and lend further support to the feasibility of passive immunotherapy for AIDS patients. J. Med. Virol. 65:649–658, 2001. © 2001 Wiley‐Liss, Inc.