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Epstein Barr virus genotypes and LMP‐1 variants in HIV‐infected patients
Author(s) -
Correa Rita Mariel,
Dolores Fellner María,
Durand Karina,
Redini Liliana,
Alonio Virginia,
Yampolsky Claudio,
Colobraro Antonio,
Sevlever Gustavo,
Teyssié Angélica,
Benetucci Jorge,
Picconi María Alejandra
Publication year - 2007
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.20782
Subject(s) - virology , genotype , biology , virus , epstein–barr virus , polymerase chain reaction , asymptomatic , gammaherpesvirinae , peripheral blood mononuclear cell , gene , lymphoma , herpesviridae , immunology , viral disease , medicine , pathology , genetics , in vitro
Abstract Two Epstein Barr virus (EBV) genotypes: EBV‐1 and EBV‐2 have been described. A 30‐bp deletion in latent membrane protein‐1 gene (del‐LMP‐1) has been identified in various pathologies. The aim of this study was to determine EBV genotypes and 30‐bp deletion frequency in HIV‐infected patients from Argentina. The study was performed on 258 individuals: Cases : 144 HIV‐infected patients that included: (a) 7 AIDS patients with primary central nervous system lymphoma (PCNSL), (b) 62 AIDS patients, and (c) 75 asymptomatic HIV‐infected patients. Controls : 114 HIV‐negative individuals. EBV genotypes and variants in LMP‐1 gene were detected by polymerase chain reaction (PCR)‐Southern blot on DNA extracted from peripheral blood mononuclear cells and brain biopsies. In PCNSL, the presence of EBV was confirmed by EBER RNA in situ hybridization, and DNA sequencing of 3′ end LMP‐l gene of PCR products was performed. In HIV‐infected patients, EBV‐1 was detected in 48.6%, EBV‐2 in 18.8%, and co‐infection with both genotypes in 32.6%. In control group, EBV‐1 was present in 74.3%, EBV‐2 in 12.4%, and co‐infection in 13.3%. Del‐LMP‐1 was found in 44.4% of HIV‐infected patients samples (20.7% alone and 23.7% co‐infection with non‐deleted form) while it was found in 25.3% (6.3% alone and 19% with co‐infection) in HIV‐negative individuals. In HIV‐infected patients EBV‐2, co‐infection and 30‐bp deletion are more prevalent than in control group. In all, PCNSL brain biopsies samples, del‐LMP‐1 always was detected with EBV‐2, but more cases would have to be included to draw definitive conclusions. J. Med. Virol. 79:401–407, 2007. © 2007 Wiley‐Liss, Inc.