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Isolation and identification of adenovirus from conjunctival scrapings over a two‐year period (between 2001 and 2003) in Yokohama, Japan
Author(s) -
Matsui Kiyohiko,
Saha Sukumar,
Saitoh Masaaki,
Mizuki Nobuhisa,
Itoh Norihiko,
Okada Eiichi,
Yoshida Atsushi,
Xin KeQin,
Nishio Osamu,
Okuda Kenji
Publication year - 2007
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.20779
Subject(s) - serotype , restriction fragment length polymorphism , virology , restriction enzyme , polymerase chain reaction , mastadenovirus , biology , neutralization , keratoconjunctivitis , adenoviridae , virus , gene , genetics , recombinant dna
Over a 2‐year period between 2001 and 2003, a total of 115 conjunctival scrapings were collected from patients with keratoconjuctivitis from several hospitals in Yokohama, Japan. Out of 115, 94 (82.4%) cases of adenoviruses were detected by polymerase chain reaction (PCR); 60 (52.1%) by cell culture isolation; and 16 (14.0%) by enzyme‐linked immunosorbent assay (ELISA). The serotypes were determined by PCR‐ restriction fragment length polymorphism analysis (PCR‐RFLP) and by the neutralization test (NT). PCR‐RFLP was performed using a combination of endonucleases such as Hha I, Alu I, and Hae III. Of the 94 PCR‐positive samples, the serotypes of 91 (96.8%) were identified by PCR‐RFLP analysis (adenovirus 3: 50%, 4: 11%, and 8: 32%). Out of the 115 samples, 60 samples were identified by the neutralization (adenovirus 3, 4, 7, and 8). When both PCR‐RFLP and the neutralization techniques were used, 53.2%, 11.7%, 1.1%, and 34% of the samples were identified as adenovirus 3, 4, 7, and 8, respectively. In contrast to the results of a nationwide surveillance report, adenovirus 3 was found as a major cause of keratoconjunctivitis in the Yokohama area. The nationwide surveillance report did not reflect accurately the epidemiological situation in the local area. In order to obtain surveillance data that would be useful for the prevention of an adenovirus conjunctivitis epidemic, it seems that local epidemiology is more important than that nationwide surveillance. J. Med. Virol. 79:200–205, 2007. © 2006 Wiley‐Liss, Inc.