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Apoptosis‐associated gene expression in HIV‐infected patients in response to successful antiretroviral therapy
Author(s) -
Balestrieri Emanuela,
Grelli Sandro,
Matteucci Claudia,
Minutolo Antonella,
d'Ettorre Gabriella,
Di Sora Fiorella,
Montella Francesco,
Vullo Vincenzo,
Vella Stefano,
Favalli Cartesio,
Macchi Beatrice,
Mastino Antonio
Publication year - 2007
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.20768
Subject(s) - fadd , tradd , apoptosis , peripheral blood mononuclear cell , virology , downregulation and upregulation , antiretroviral therapy , genetic enhancement , programmed cell death , immunology , caspase 8 , human immunodeficiency virus (hiv) , fas receptor , biology , gene , lentivirus , gene expression , medicine , caspase 3 , caspase , viral load , viral disease , death domain , genetics , in vitro
The simultaneous expression of 19 apoptosis‐related genes was analyzed by RNA‐protection assay in peripheral blood mononuclear cells of HIV‐infected patients before and during successful antiretroviral therapy (ART). After 12 months of therapy, the expression of the pro‐apoptotic genes FAS , FAS‐L , FAF‐1 , FADD , CASPASE‐8 , DR3 , TRAIL , TNFR‐1 , TRADD , and BAX was significantly downregulated with respect to time 0, while that of BCL‐2 , BCL‐XL , and MCL‐1 was significantly upregulated. The data suggest that inhibition of cell death in HIV‐positive patients under successful therapy is the result of a complex network of multifactor signaling, correlated with both death and survival of lymphocytes. J. Med. Virol. 79:111–117, 2007. © 2006 Wiley‐Liss, Inc.