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Identification of NV‐F virus DNA in hepatocellular carcinoma
Author(s) -
Yeh ChauTing,
Chen TseChing,
Chang MingLing,
Hsu ChaoWei,
Yeh TaSen,
Lee WeiChen,
Huang ShiuFeng,
Tsai ChihChieh
Publication year - 2007
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.20763
Subject(s) - virus , hepatocellular carcinoma , virology , hepatitis b virus , dna virus , dna , biology , cirrhosis , hepatitis c virus , medicine , gene , genome , cancer research , genetics , gastroenterology
A fragment of DNA sequence derived from a hepatotropic virus, named NV‐F was isolated recently. The aim of this study was to examine whether this virus was associated with hepatocellular carcinoma (HCC). Total cellular DNA was extracted from hepatocellular carcinoma tissues. NV‐F virus DNA was detected by PCR. The PCR products were subjected to sequence analysis. Of the 78 HCC samples included, 12 (15.4%) were positive for NV‐F virus DNA. Sequence analysis of the 12 amplified DNA fragments revealed a point mutation in one of them. The clinicopathological parameters between patients with and without NV‐F virus infection were compared. It was found that patients with NV‐F virus infection were older than those without NV‐F virus infection (mean ages, 61.5 versus 52.5 years; P = 0.032). Otherwise, no difference was observed between the two groups. Of the 12 HCC patients positive for NV‐F virus DNA, 11 patients were co‐infected by either hepatitis B or C virus. The remaining patient was a Taiwanese aboriginal inhabitant with cryptogenic cirrhosis. In conclusion, NV‐F virus DNA was identified in 15.4% of HCC tissues. HCC patients with NV‐F virus infection were significantly older than those without NV‐F virus infection. J. Med. Virol. 79:92–96, 2007. © 2006 Wiley‐Liss, Inc.