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Bone marrow transplantation from a pediatric donor with a high frequency of cytomegalovirus‐specific T‐cells
Author(s) -
Komatsu Haruki,
Kogawa Kazuhiro,
oyama Shigeaki,
Inui Ayno,
Sogo Tsuyoshi,
Fujisawa Tomoo,
Klenerman Paul
Publication year - 2006
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.20746
Subject(s) - cd8 , transplantation , immunology , cytotoxic t cell , t cell , bone marrow , biology , virology , medicine , immune system , in vitro , biochemistry
A recent study reported that quantitation of cytomegalovirus (CMV)‐specific CD8+ T lymphocytes in the graft and monitoring of these T cells might identify hematopoietic stem cell transplantation‐recipients at the risk for progressive CMV infection. A 6‐year‐old girl underwent bone marrow transplantation from an HLA‐identical sibling with a very high frequency of CMV specific tetramer‐positive CD8+ T‐cells. CMV‐specific T‐cell immunity was prospectively evaluated using a peptide (HLA‐A2, NLVPMVATV). Tetramer assay showed that the frequency of CMV‐specific CD8+ T cells of the donor in the peripheral blood was 5.3%, higher than average amongst young children. The frequency of CMV‐specific CD8+ T cells of the donor in the graft was 3.7% of CD8+ T‐cells. Before transplantation, the frequency of CMV specific CD8+ T cells of the recipient was 0.1% in the peripheral blood. Surprisingly, the frequency of CMV specific CD8+ T cells increased up to 30% of CD8+ T‐cells at day 27 after transplantation. IFN‐γ enzyme‐linked immunospot assay showed the recipient‐T cells had strong responses to the A2‐specific NLVPMVATV peptide. Although the phenotypic pattern of the CMV‐specific T cells of the recipient was different from those of the donor before transplantation, the phenotype of the donor‐derived cells retained their original phenotype in the recipient after transplantation. These finding suggested that active transferred immunity from the graft with a high frequency of CMV‐specific CTL could induce a rapid reconstitution of CMV‐specific T‐cell mediated immunity in pediatric HLA‐identical allogenetic bone marrow transplantation. The screening of peripheral blood using HLA‐peptide tetramer staining might be beneficial to select donors. J. Med. Virol. 78:1616–1623, 2006. © 2006 Wiley‐Liss, Inc.