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Detection of herpes simplex virus type 1 DNA in bilateral human trigeminal ganglia and optic nerves by polymerase chain reaction
Author(s) -
Motani Hisako,
Sakurada Koichi,
Ikegaya Hiroshi,
Akutsu Tomoko,
Hayakawa Mutsumi,
Sato Yayoi,
Yajima Daisuke,
Sato Kaoru,
Kobayashi Kazuhiro,
Iwase Hirotaro
Publication year - 2006
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.20742
Subject(s) - herpes simplex virus , trigeminal ganglion , polymerase chain reaction , virology , trigeminal nerve , virus , dna , hsl and hsv , biology , herpesviridae , microbiology and biotechnology , alphaherpesvirinae , medicine , anatomy , viral disease , gene , genetics , sensory system , neuroscience
Herpes simplex virus type 1 (HSV‐1) DNA was extracted from human trigeminal ganglia of 121 cadavers aged between 3 months and 94 years, and its PCR amplification was performed for the RL2 HSV‐1 sequence using two pairs of primers. The HSV‐1 DNA was detected in 74 of 121 patients (61%); 70/74 bilaterally, 3/74 only on the left side, and 1/74 only on the right side. Although the PCR‐positive rate was significantly higher in advanced age, the correlation between the PCR‐positive rate and gender was unclear. Additionally, HSV‐1 DNA was not detected in any of the 50 optic nerve samples. J. Med. Virol. 78:1584–1587, 2006. © 2006 Wiley‐Liss, Inc.