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New genome type of adenovirus serotype 19 causing nosocomial infections of epidemic keratoconjunctivitis in Japan
Author(s) -
TanakaYokogui Keico,
Itoh Norihiko,
Usui Norio,
Takeuchi Satoshi,
Uchio Eiichi,
Aoki Koki,
Usui Masahiko,
Ohno Shigeaki
Publication year - 2001
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.2068
Subject(s) - restriction enzyme , virology , serotype , keratoconjunctivitis , biology , genome , mastadenovirus , adenoviridae , microbiology and biotechnology , dna , genetics , gene , recombinant dna
Twelve strains of adenovirus serotype 19, isolated from cases of epidemic keratoconjunctivitis in Japan in 1992, 1993, 1997, and 1998, were analyzed by DNA restriction analysis, using restriction endonucleases Bam HI, Bgl I, Bgl II, Eco RI, Hin dIII, Kpn I, Pst I, Sac I, Sal I, Sma I, and Xho I. Among these 11 restriction endonucleases, Eco RI, Pst I, Sac I, and Sma I were discriminative enzymes, showing restriction patterns different from those reported previously for the prototype and the variant 19a. This new genome type was isolated in 1997 and 1998, when an increase of epidemic keratoconjunctivitis cases caused by adenovirus serotype 19 was observed for both sporadic and nosocomial infections. Strains from 1992 and 1993 showed restriction patterns similar to those of the worldwide reported variant 19a for all enzymes used. The changes detected in strains from 1997 and 1998 could be the reason for the recent epidemic. J. Med. Virol. 65:530–533, 2001. © 2001 Wiley‐Liss, Inc.