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Molecular characterization of a Canadian human parechovirus (HPeV)‐3 isolate and its relationship to other HPeVs
Author(s) -
Abed Yacine,
Boivin Guy
Publication year - 2005
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.20493
Subject(s) - capsid , biology , virology , genome , phylogenetic tree , genetics , gene , virus
A new member of the Parechovirus genus (HPeV‐3) has been recently isolated from a stool specimen of a young Japanese child with transient paralysis (isolate A308/99). Three additional HPeV‐3 Canadian cases associated with severe neonatal infections were subsequently described by our group. At this time, limited information is available on the molecular characterization of the HPeV‐3 genome. In this study, we report the complete genome sequence of a Canadian HPeV‐3 isolate (Can82853‐01) recovered in 2001 from the nasopharyngeal aspirate of a neonate with a sepsis‐like syndrome. The Can82853‐01 genome was 7,322 nucleotides (nt) in length and had 96.4%, 77.7%, and 77.5% nt identity with the A308/99 (HPeV‐3), HPeV‐1, and HPeV‐2 reference strains, respectively. Furthermore, the Can82853‐01 polyprotein had 98.2%, 86.9%, and 84.7% amino acids identity compared to the same viruses. Most differences between the polyproteins of HPeV‐3 isolates and those of HPeV‐1 and ‐2 were located in the VP0‐VP3‐VP1 capsid region whereas the non‐structural proteins were relatively conserved. The primary and predicted secondary structures of 5′UTR and 3′UTR of the HPeV‐3 isolates were also different than those of HPeV‐1 and ‐2 reference strains. Phylogenetic analysis confirmed the relatedness between Can82853‐01 and A308/99 and their divergence from other picornaviruses. J. Med. Virol. 77:566–570, 2005. © 2005 Wiley‐Liss, inc.