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Cytomegalovirus and human herpesvirus 8 DNA detection in peripheral blood monocytic cells of AIDS patients: Correlations with the presence of Kaposi's sarcoma and CMV disease
Author(s) -
van der Kuyl Antoinette C.,
Polstra Abeltje M.,
van den Burg Remco,
Jan Weverling Gerrit,
Goudsmit Jaap,
Cornelissen Marion
Publication year - 2005
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.20396
Subject(s) - betaherpesvirinae , cytomegalovirus , virology , herpesviridae , peripheral blood mononuclear cell , human cytomegalovirus , viral disease , medicine , immunology , kaposi's sarcoma , virus , viral load , sarcoma , disease , biology , human herpesvirus , pathology , biochemistry , in vitro
To establish the effect of the presence in blood cells of cytomegalovirus (CMV) and human herpesvirus 8 (HHV8) DNA, two herpesviruses that are activated frequently in AIDS patients, were selected from the Amsterdam Cohort Studies on HIV/AIDS 181 PBMC samples from patients with and without Kaposi's sarcoma (KS), and with and without CMV‐related disease. The viral loads of both HHV8 and CMV were determined by real‐time PCR at the time of diagnosis of AIDS. There was no significant difference in prevalence and load for CMV between the KS and non‐KS patients. The variable related most strongly to KS was the presence of HHV8 DNA in PBMCs, whilst CMV DNA was related to the development of CMV disease and shortened survival. The frequency of detection of HHV8 increased when the patient presented with more severe KS symptoms at diagnosis, but detection of HHV8 DNA did not influence survival. Therefore, HHV8 and CMV DNA measured in the blood of AIDS patients, are each related mainly to the associated disease, and have no additional predictive value in these patients. J. Med. Virol. 76:541–546, 2005. © 2005 Wiley‐Liss, Inc.