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Expression and antigenicity of virus‐like particles of norovirus and their application for detection of noroviruses in stool samples
Author(s) -
Kamata Kunio,
Shinozaki Kuniko,
Okada Mineyuki,
Seto Yoshiyuki,
Kobayashi Shinichi,
Sakae Kenji,
Oseto Mitsuaki,
Natori Katsuro,
ShiratoHorikoshi Haruko,
Katayama Kazuhiko,
Tanaka Tomoyuki,
Takeda Naokazu,
Taniguchi Koki
Publication year - 2005
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.20334
Subject(s) - norovirus , capsid , virology , caliciviridae , antigenicity , biology , virus , norwalk virus , genotype , recombinant dna , antigen , microbiology and biotechnology , gene , genetics
Human noroviruses (NoVs), members of the genus Norovirus in the family Caliciviridae , are the leading agents of nonbacterial acute gastroenteritis worldwide. Human NoVs are currently divided into at least two genogroups, genogroup I (GI) and genogroup II (GII), each of which contains at least 14 and 17 genotypes. To explore the genetic and antigenic relationship among NoVs, we expressed the capsid protein of four genetically distinct NoVs, the GI/3 Kashiwa645 virus, the GII/3 Sanbu809 virus, the GII/5 Ichikawa754 virus, and the GII/7 Osaka10‐25 virus in baculovirus expression system. An antigen enzyme‐linked immunosorbent assay (ELISA) with hyperimmune serum against the four recombinant capsid proteins and characterized previously three capsid proteins derived from GI/1, GI/4, and GII/12 was developed to detect the NoVs antigen in stools. The antigen ELISA was highly specific to the homotypic strains, allowing assignment of a strain to a Norovirus genetic cluster within a genogroup. J. Med. Virol. 76:129–136, 2005. © 2005 Wiley‐Liss, Inc.