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Internalization of the dengue virus is cell cycle modulated in HepG2, but not vero cells
Author(s) -
Phoolcharoen Waranyoo,
Smith Duncan R.
Publication year - 2004
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.20195
Subject(s) - permissiveness , vero cell , virology , dengue virus , biology , serotype , dengue fever , virus , internalization , cell , antibody dependent enhancement , cell culture , microbiology and biotechnology , viral replication , genetics
While many studies have investigated the relationship between cell type and dengue virus infection, no study to date has examined the effect of cell physiology on permissiveness to infection. Unsynchronized and artificially synchronized cell populations at different stages of the cell cycle of two cell types (Vero and HepG2) were examined for permissiveness to infection by two dengue virus serotypes (serotypes 2 and 3) by determining both the levels of virus produced as well as the percentage of cells infected. Vero cells showed no significant differences between either viral production or percentage of cells infected as compared to unsynchronized cells for any of the phases investigated, although production of virus (for both serotypes 2 and 3) was somewhat lower for cells infected during S phase. In contrast, HepG2 cells were significantly more permissive for both infection and virus production in the G 2 phase as compared to other phases examined and serotype differences in permissiveness to infection were noted with cells in the M phase of the cell cycle. These results suggest that the cell cycle may be a mediator of cell permissiveness in some cell types. J. Med. Virol. 74:434–441, 2004. © 2004 Wiley‐Liss, Inc.