Premium
Antigenic alteration of influenza B virus associated with loss of a glycosylation site due to host‐cell adaptation
Author(s) -
Saito Takehiko,
Nakaya Yoko,
Suzuki Takashi,
Ito Reiko,
Saito Toshinori,
Saito Hiroyuki,
Takao Shinichi,
Sahara Keiji,
Odagiri Takato,
Murata Takeomi,
Usui Taichi,
Suzuki Yasuo,
Tashiro Masato
Publication year - 2004
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.20178
Subject(s) - biology , virology , embryonated , glycosylation , sialic acid , virus , hemagglutinin (influenza) , immunogenicity , epitope , antigen , microbiology and biotechnology , host adaptation , antigenic drift , neuraminidase , viral replication , serial passage , virulence , gene , genetics
Abstract Effects of host‐cell adaptation of the hemagglutinin (HA) protein were evaluated by the analyses of four pairs of recent influenza B field isolates, each pair consisting of an Madin Darby canine kidney (MDCK)‐ and an embryonated chicken egg‐derived isolates from the same clinical specimen. Among the isolates examined, all of the MDCK‐derived isolates retained glycosylation site at amino acid 197 on the HA1 molecule, whereas three egg‐derived isolates lost it. Antigenic difference in the HA molecule between an MDCK‐ and an egg‐derived isolates of three of these pairs was demonstrated to be associated with the glycosylation 197. Replication of the MDCK‐derived isolates was suppressed in eggs, suggesting that the presence of the glycosylation 197 was disadvantageous to replication in eggs. Virus‐binding affinity assay revealed that the loss of carbohydrate chain did not significantly alter the preferential recognition of sialic acid linkage. Immunogenicity and vaccine efficacy of an MDCK‐ and an egg‐derived clones of B/Akita/27/2001, the former retained the glycosylation 197 and the latter lost it, were compared in a hamster model. When formalin‐inactivated whole virion vaccines prepared from the paired isolates were administered into hamsters, no significant difference between them was observed in protective ability against challenges by the homologous and heterologous clones. Implication of the egg adaptation of influenza virus to antigenic surveillance of the field isolates as well as the selection of vaccine strains, and possibility of the involvement of the viral protein(s) other than the HA in the egg adaptation were discussed. J. Med. Virol. 74:336–343, 2004. © 2004 Wiley‐Liss, Inc.