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I. hepatitis E virus (87A strain) propagated in A549 cells
Author(s) -
Huang Rutong,
Nakazono Naoki,
Ishii Keizo,
Li Derong,
Kawamata Osamu,
Kawaguchi Ryuji,
Tsukada Yutaka
Publication year - 1995
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890470402
Subject(s) - virology , strain (injury) , hepatitis a virus , virus , biology , anatomy
A strain of hepatitis E virus (HEV), the 87A strain isolated in 2BS cells from the feces of a patient with hepatitis E, has been reported previously. In this study, the 87A strain was propagated in A549 cells, and the marked cytopathic effect (CPE) appeared in the infected monolayer cells. The size of this virus is about 30 nm in diameter. Furthermore, HEV‐RNAfrom the supernatants of the virus of different passages was detected by polymerase chain reaction (PCR) amplification using ET1 .1 HEV primers. A band of HEV for 239 bp from PCR products was revealed by electro‐phoresis. PCR products of the fourth passage were sequenced. These results show that the 87A virus replicates in the A549 cell line. © Wiley‐Liss, Inc.