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Reverse transcriptase mutations in sequential HIV‐1 isolates in a patient with AIDS
Author(s) -
Gurusinghe A. D.,
Land S. A.,
Birch C.,
McGavin C.,
Hooker D. J.,
Tachedjian G.,
Doherty R.,
Deacon N. J.
Publication year - 1995
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890460312
Subject(s) - reverse transcriptase , virology , zidovudine , mutation , human immunodeficiency virus (hiv) , virus , biology , polymerase chain reaction , gene , lentivirus , sequence (biology) , viral disease , sequence analysis , polymerase , genetics
Sequential human immunodeficiency virus type 1 (HIV‐1) isolates were obtained over a 29‐month period from a person before, during, and after AZT therapy. DNA sequence analysis of polymerase chain‐amplified reverse‐transcriptase gene showed a gradual accumulation of mutations to peak resistance (lC 50 2.13 μM AZT) in association with mutations at codons 44, 210, and 369, as well as at 41, 67, 70, and 215. Eight months after cessation of AZT therapy, when an HIV‐1 isolate from the patient was again sensitive to AZT, these mutations had all returned to the pretherapy sequence. © 1995 Wiley‐Liss, Inc.