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Isolation of black creek canal virus, a new hantavirus from Sigmodon hispidus in Florida
Author(s) -
Rollin Pierre E.,
Ksiazek Thomas G.,
Elliott Luanne H.,
Ravkov Eugene V.,
Martin Mary Lane,
Morzunov Sergey,
Livingstone Walter,
Monroe Marty,
Glass Gregory,
Ruo Sup,
Khan Ali S.,
Childs James E.,
Nichol Stuart T.,
Peters C. J.
Publication year - 1995
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890460108
Subject(s) - sigmodon hispidus , hantavirus , virology , biology , virus , cotton rat , bunyaviridae , hantavirus pulmonary syndrome , serology , antibody , immunology , zoology
Numerous rodents were trapped for serologic and virologic studies following the identification of a hantavirus pulmonary syndrome (HPS) case in Dade County, Florida. Cotton rats ( Sigmodon hispidus ) were the most frequently captured rodent and displayed the highest seroprevalence to a variety of hantavirus antigens. Hantavirus genome RNA was detected in all the seropositive cotton rats tested, using a reverse transcriptase‐polymerase chain reaction (RT‐PCR) assay. A virus was isolated from tissues of two seropositive cotton rats by cultivation of lung and spleen homogenates on Vero E6 cells. Nucleotide sequence information obtained by direct RT‐PCR and the serologic relationships of this virus with the other hantaviruses indicate that this virus, Black Creek Canal virus, represents a new hantavirus distinct from the previously known serotypes. © 1995 Wiley‐Liss, Inc.