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Human papillomavirus‐16 and ‐18 replication in esophagus squamous cancer cell lines does not require sheterologous E1 and E2 proteins
Author(s) -
and Kazumi Togawa,
Rustgi Anil K.
Publication year - 1995
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890450414
Subject(s) - virology , biology , cell culture , transfection , viral replication , esophagus , heterologous , gene , cancer research , virus , microbiology and biotechnology , genetics , anatomy
Human papillomaviruses (HPVs) are doublestranded DNA viruses that replicate in the nuclei of squamous epithelial cells. HPVs can be classified into high‐risk (e.g., types 16, 18, 31, and 33) or low‐risk (e.g., types 6, 11, and 30), depending on their association with benign or malignant tumors. We recently described the association of HPV‐16 and ‐18 with esophagus squamous cell cancer. HPV replication was studied in representative cell lines derived from esophagus cancers. HPV‐16 and ‐18 genomes were independently transiently transfected into HCE‐4 and HCE‐7 cell lines with and without E1 and E2 genes under heterologous promoters. Southern blot analysis demonstrated that these cell lines support viral replication. However, heterologous E1 and E2 are not required for HPV replication. These findings suggest that specific host nuclear factors in esophageal squamous epithelial cells may support HPV replication. © 1995 Wiley‐Liss, Inc.