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Herpes simplex virus‐1 replication in histiotypic rotation‐mediated reaggregated murine brain
Author(s) -
Fowler Sandra L.,
Forbes Robert A.
Publication year - 1995
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890450409
Subject(s) - virology , herpes simplex virus , titer , viral replication , biology , virus , permissiveness , inoculation , plaque forming unit , cell culture , microglia , microbiology and biotechnology , immunology , genetics , inflammation
Inbred mouse strains exhibit varying susceptibilities to severe herpes simplex virus (HSV)‐1 ‐related neurologic disease. HSV‐1 replication was examined in neural tissue obtained from mouse strains susceptible (A/J, SJL), moderately resistant (Balb/c), or resistant (C57BL/6) to severe HSV‐1 disease. Reaggregated brain cultures were prepared from mechanically dissociated fetal mouse brains maintained with constant rotation. The resulting aggregates each contain neurons, astrocytes, oligodendrocytes, and microglia. These were inoculated with 10 −2 −10 4 plaque‐forming units (pfu) HSV‐1 Maclntyre/aggregate. Aggregates and media were harvested at 24, 48, 72, and 96 hr post‐inoculation (p.i.) and assayed for virus production by plaque titration. Brain cultures prepared from A/J, SJL, Balb/c, and C57BL/6 mice supported HSV‐1 replication equally well: by 96 hr p.i., titers of 10 6 pfu/ml were produced by each strain at each inoculum. ID 50 s were similar for A/J and C57BL/6 cultures. There was no increased capacity for HSV‐1 replication or for permissiveness for HSV‐1 infection in histiotypic brain cultures from mouse strains susceptible to severe HSV‐1 disease. © 1995 Wiley‐Liss, Inc.