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Expression of the Epstein‐Barr virus DNA polymerase in Escherichia coli for use as antigen for the diagnosis of nasopharyngeal carcinoma
Author(s) -
Lin LungShen,
Ro LeeHwa,
Lo MuShung,
Huang WanLing,
Ma Jeng,
Chang TongHsuan,
Shu ChihHung,
Chow KuanChih,
Liu WuTse,
Chen Kuang Y.,
Yang HueyLang
Publication year - 1995
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890450118
Subject(s) - virology , biology , virus , dna polymerase , nasopharyngeal carcinoma , recombinant dna , antibody , herpes simplex virus , microbiology and biotechnology , western blot , epstein–barr virus , antigen , herpesviridae , polymerase , dna , gene , immunology , viral disease , medicine , biochemistry , genetics , radiation therapy
Epstein‐Barr virus (EBV) encoded DNA polymerase (POL) was cloned and over‐expressed in Escherichia coli . Western blot analysis confirmed the presence of antibody to this POL protein in sera from nasopharyngeal carcinoma (NPC) patients. By Western blot analysis, moderate to high concentration of IgG POL‐specific antibodies were present in 43 of 48 NPC sera and only 4 of 48 healthy, seropositive controls. The POL‐specific IgG antibodies appear as early as stage I of NPC, suggesting that the recombinant POL protein can be a useful diagnostic marker for early diagnosis of the disease. It was also found that human sera containing high titer of cytomegalovirus (CMV) antibodies or herpes simplex virus type 1 (HSV‐1) antibodies did not cross‐react with the recombinant EBV POL, despite the homology shared by DNA polymerase proteins of these viruses. © 1995 Wiiey‐Liss, inc.