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Nucleotide sequence of the core region of hepatitis C virus in Pakistan and Bangladesh and the geographic characterisation of hepatitis C virus in South Asia
Author(s) -
Ohno Tomoyoshi,
Mizokami Masashi,
Tibbs Christopher J.,
NouriAria Kayhan T.,
Wu RongRong,
Ohba KenIchi,
Orito Etsuro,
Suzuki Kaoru,
Mizoguchi Naoto,
Nakano Tatsunori,
Khan Mobin,
Yano Michitani,
Kiyosawa Kendo,
Williams Roger
Publication year - 1994
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890440409
Subject(s) - virology , genotype , hepatitis c virus , biology , flaviviridae , homology (biology) , virus , nucleic acid sequence , sequence analysis , hepacivirus , molecular epidemiology , genetics , gene
A large number of complete and partial hepatitis C virus (HCV) sequences have been reported and classified into several genotypes, although none have been reported from South Asia. We have determined and evaluated partial sequences in the core region of HCV obtained from patients with chronic hepatitis in Pakistan and Bangladesh. Nucleotide sequences from these viruses show significant homology with the Japanese HCV‐TR isolate (91.7%–97.9%) and low homology with other Japanese, American, and UK isolates including HCV‐1, HC‐J4, HC‐J6, HC‐J8, and E‐b1 (79.3%–86.2%). The homologies of their deduced amino acids sequence with HCV‐1, HC‐J4, HC‐J6, HC‐J8, E‐b1, and HCV‐TR were 84.3%–89.8%, 85.0–87.9%, 84.1%–86.9%, 84.3%–87.0%, 90.2%–93.1%, and 89.8%–93.5%, respectively. These results suggest that our clones might be classified into the same genotype as HCV‐TR. Further analysis using molecular evolutionary methods strongly supported the classification of these sequences with the HCV‐TR genotype. Moreover, we could not detect any isolates which were closely related to our clones or HCV‐TR in countries outside the South Asian area. These data further support the association of HCV genotypes with distinct geographic regions. © 1994 Wiley‐Liss, Inc.