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Molecular epidemiology of enterovirus outbreaks in Canada during 1991–1992: Identification of echovirus 30 and coxsackievirus B1 strains by amplicon sequencing
Author(s) -
Drebot Michael A.,
Nguan Christopher Y.,
Campbell Janice J.,
Lee Spencer H. S.,
Forward Kevin R.
Publication year - 1994
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890440406
Subject(s) - echovirus , amplicon , virology , enterovirus , outbreak , biology , coxsackievirus , serotype , molecular epidemiology , nucleic acid sequence , sequence analysis , genetics , genotype , virus , polymerase chain reaction , dna , gene
The relatedness of enteroviral isolates associated with two recent outbreaks in Canada was assessed using direct sequencing of amplicons derived from a large portion of the 5′ nontrans‐lated region (NTR) of the viral genome. The amplicons of 60 echovirus 30 isolates originating from seven different provinces in 1991 were found to share 99% or greater sequence identity. Recent coxsackievirus B1 isolates characterised in the same manner were identical to each other. When the 5′ NTR sequence of these isolates was compared to prototype strains a difference of 11–15% in nucleotide composition was observed. These results indicate that the variability of nucleotide sequence found in 5′ NTRs can be utilized to identify rapidly enteroviral strains associated with particular outbreaks and distinguish them from other strains and serotypes. © 1994 Wiley‐Liss, Inc.