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Simple procedure for discrimination and typing of enteric adenoviruses after detection by polymerase chain reaction
Author(s) -
Allard Annika,
Kajon Adriana,
Wadell Göran
Publication year - 1994
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890440307
Subject(s) - virology , polymerase chain reaction , enteric virus , typing , simple (philosophy) , biology , microbiology and biotechnology , genetics , gene , philosophy , epistemology
Abstract A procedure was developed for specific discrimination and typing of the enteric human adenoviruses, Ad40 and Ad41, after polymerase chain reaction (PCR) amplification of a sequence in the hexon gene highly conserved among all 47 sero‐types recognised. By Taq I restriction of the 300 bp amplimers, subgenus F DNA could be discriminated from DNAs of adenoviruses belonging to all other subgenera. Discrimination between Ad40 and Ad41 was subsequently achieved by cleavage with either Cfo I, HinP I, Mae III, Mvn I, and/or Rsa I. Thus, PCR detection of viral DNA combined with restriction analysis of amplified products provides a valuable tool for use in epidemiological studies of diarrhoea of adenoviral aetiology. © 1994 Wiley‐Liss, inc.