Premium
Presence of infective Epstein‐Barr virus in the urine of patients with infectious mononucleosis
Author(s) -
Landau Zvi,
Gross Raz,
Sanilevich Anka,
Friedmann Adam,
MitraniRosenbaum Stella
Publication year - 1994
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890440303
Subject(s) - urine , mononucleosis , virology , virus , polymerase chain reaction , epstein–barr virus , herpesviridae , medicine , viral disease , immunology , biology , gene , biochemistry
The presence of Epstein‐Barr virus (EBV) in the blood and urine of 20 patients with infectious mononucleosis (IM) was investigated together with the clinical course of the disease, and in 9 patients up to 2–7 months after recovery. EBV DNA, analyzed by the polymerase chain reaction (PCR), was detected in the blood of all 20 patients from the first sample obtained and detected between 3 to 42 days from the beginning of symptoms and up to 2–3 months after recovery. In the urine, EBV DNA was detected in 15 out of 16 (93%) patients in the first sample obtained and detected between 3 to 50 days during the clinical course of the disease. In four patients EBV DNA was detected in the urine up to 3 months after full recovery. Seventeen out of 26 (65%) urine samples including 3 which were obtained 2–7 months after recovery infected B cells as assessed by PCR. Nine out of 12 (75%) urine samples tested induced Epstein‐Barr nuclear antigen (EBNA) in the infected B‐cell line. In addition to the persistence of EBV in the blood of IM patients, these studies show for the first time the presence of infective EBV in the urine during the clinical course of the disease and up to 7 months after full clinical recovery. © 1994 Wiley‐Liss, Inc.