Premium
Serotyping of human rotaviruses in the Tokyo area (1990–1993) by enzyme immununoassay with monoclonal antibodies and by reverse transcription and polymerase chain reaction amplification
Author(s) -
Ushijima Hiroshi,
Mukoyama Atsushi,
Hasegawa Ayako,
Nishimura Shuichi,
Konishi Kyoko,
Bosu Kim
Publication year - 1994
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890440208
Subject(s) - virology , monoclonal antibody , reverse transcriptase , serotype , polymerase chain reaction , rotavirus , reverse transcription polymerase chain reaction , biology , microbiology and biotechnology , immunoassay , virus , polymerase , antibody , gene , messenger rna , genetics
Serotyping of human rotavirus in the Tokyo area was conducted from 1990 to 1993 by enzyme immunoassay with monoclonal antibodies (EIAMAbs) against VP7 and by reverse transcription and polymerase chain reaction (RT‐PCR) amplification of the VP4 and VP7 genes. The results by EIA‐MAbs were very similar to those obtained by RT‐PCR. Evidence of intraserotypic variations was suggested because strains of undetermined serotypes were detected by either EIA‐MAb or RT‐PCR. This kind of study is required for vaccine development. © 1994 Wiley‐Liss, Inc.