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Rapid detection of virus genome from imported dengue fever and dengue hemorrhagie fever patients by direct polymerase chain reaction
Author(s) -
Morita Kouichi,
Maemoto Tatsuo,
Honda Shoji,
Onishi Kenji,
Murata Misako,
Tanaka Mariko,
Igarashi Akira
Publication year - 1994
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890440111
Subject(s) - dengue fever , virology , dengue virus , polymerase chain reaction , virus , flaviviridae , reverse transcription polymerase chain reaction , biology , reverse transcriptase , polymerase , virus isolation , genome , viral disease , gene , genetics , messenger rna
Serum specimens from patients with imported dengue fever and dengue hemorrhagic fever were directly subjected to reverse transcription and polymerase chain reaction (RT‐PCR) without any RNA purification. The amplified virus genome was detected within 3 hours. The results of PCR corresponded closely to the results of virus isolation using cultured mosquito cells, suggesting that direct RT‐PCR procedure greatly facilitates rapid diagnosis of dengue infection. © 1994 Wiley‐Liss, Inc.