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Human papillomavirus DNA and anti‐HPV secretory IgA antibodies in cytologically normal cervical specimens
Author(s) -
Veress György,
Konya József,
CsikyMészáros Tibor,
Czeglédy Judit,
Gergely Lajos
Publication year - 1994
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890430219
Subject(s) - antibody , polymerase chain reaction , virology , antigen , primer (cosmetics) , hpv infection , biology , human papillomavirus , medicine , gene , immunology , cervical cancer , chemistry , cancer , genetics , organic chemistry
Cervical specimens collected from 163 cytologically healthy women were screened for the presence of human papillomavirus (HPV) DNA and anti‐HPV secretory IgA antibodies. HPV DNA was detected by a general primer mediated polymerase chain reaction (PCR), which amplifies a conserved region from the L1 ORF of genital HPVs. The PCR products were typed by restriction enzyme digestion. A total of 35 samples (21.5%) were positive for HPV DNA (13 samples for HPV 6, 6 for HPV 16, 3 for HPV 18, and 13 for untypeable HPV X). HPV DNA positivity was significantly higher among women under 25 years of age (34.8%) than among the older patients (12.4%) ( P < 0.001). An enzyme‐linked immunosorbent assay (ELISA) using synthetic peptide antigens was carried out to detect local secretory IgA antibodies against the following HPV specific antigens: HPV 16 E2, HPV 16 E7, HPV 16 L1, HPV 16 L2, and HPV 11 L2. Thirty‐four secretions (20.9%) were found to react with at least one of the oligopeptides. Anti‐HPV IgA positivity was the highest among women aged 25–32 years, and it was significantly lower in both the younger and the older age groups ( P < 0.05). Correlation between HPV DNA and anti‐HPV IgA detection was rather weak: anti‐peptide IgA positivity was 34.3% (12 of 35) among HPV DNA positive patients compared to 17.2% (22 of 128) among HPV DNA negative women ( P < 0.05). The fluctuating course of latent HPV infections should be considered in evaluating the low level of correlation between HPV DNA and anti‐HPV IgA positivity. © 1994 Wiley‐Liss, Inc.