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Detection of the negative strand of hepatitis e virus rna in the livers of experimentally infected rhesus Monkeys: Evidence for viral replication
Author(s) -
Nanda Santosh Kumar,
Panda Subrat Kumar,
Durgapal Hemlata,
Jameel Shahid
Publication year - 1994
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890420306
Subject(s) - virology , biology , virus , hepatitis e virus , viral replication , rna , rna virus , reverse transcriptase , gene , genotype , genetics
Hepatitis E virus (HEV), the causative agent of enteric non‐A, non‐B hepatitis, is a positive‐stranded RNA virus. Because of the virus's inability to grow in culture, several nonhuman primates have been used for the propagation of HEV. Using strand‐specific reverse transcription‐polymerase chain reaction (RT‐PCR), we demonstrate the presence of negative‐stranded HEV RNA replicative intermediates in the livers of infected animals. This constitutes the first direct evidence of HEV replication in the liver of the infected animals and reinforces the validity of such a model to study HEV infection, disease pathogenesis, and immunity. © 1994 Wiley‐Liss, Inc.