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Sequence variation within the human immunodeficiency virus V3 loop at seroconversion
Author(s) -
AitKhaled Mounir,
Emery Vincent C.
Publication year - 1993
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890410403
Subject(s) - viral quasispecies , virology , v3 loop , biology , seroconversion , polymerase chain reaction , virus , sequence (biology) , viral disease , antibody , dna , provirus , peripheral blood mononuclear cell , sequence analysis , nucleic acid sequence , genetics , peptide sequence , gene , genome , hepatitis c virus , in vitro
Analysis of the HIV‐1 V3 quasispecies present in an individual at the time of seroconversion was carried out. The polymerase chain reaction (PCR) was used to amplify proviral HIV‐1 DNA extracted from peripheral blood mononuclear cells from a patient who was viraemic (p24 = 15 pg/ml) and had an equivocal HIV‐1 antibody status. The PCR products were cloned and the DNA sequence determined for 15 clones. These data showed that the V3 region contained only limited sequence heterogeneity with a major variant accounting for 66% of the protein quasispecies present. The protein sequence of the principal neutralising domain on all species contained the relatively rare GPGKTL motif rather than GPGRAF. The relevance of these data for early stages of HIV infection are discussed.