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Detection of low level of human papilloma virus type 16 DNA sequences in cancer cell lines derived from two well‐differentiated nasopharyngeal cancers
Author(s) -
Huang EngShang,
Gutsch David,
Tzung KehWeei,
Lin ChinTarng
Publication year - 1993
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890400314
Subject(s) - nasopharyngeal carcinoma , virology , polymerase chain reaction , biology , dna , virus , cell culture , papilloma , cancer , human papilloma virus , nucleic acid sequence , sequence analysis , microbiology and biotechnology , gene , genetics , cervical cancer , medicine , pathology , radiation therapy
Human papilloma virus type 16 (HPV16) related sequences were detected in two EBV‐negative nasopharyngeal carcinoma (NPC) cell lines derived from two well‐differentiated NPC by polymerase chain reaction. E2 and E6 related sequences of HPV16 were demonstrated using two pairs of primers derived from these two regions. DNA sequence analysis of amplified products excluded the possibility of laboratory viral DNA contamination. © 1993 Wiley‐Liss, Inc.