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Cytomegalovirus‐specific in vitro antibody production by peripheral blood lymphocytes from renal transplant recipients with CMV infection
Author(s) -
Segondy Michel,
Barat Laurent,
Mourad Georges,
Huguet MarieFrance,
Serre Arlette,
Vendrell JeanPierre
Publication year - 1993
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890400306
Subject(s) - virology , cytomegalovirus , renal transplant , in vitro , antibody , betaherpesvirinae , immunology , cytomegalovirus infection , peripheral blood , medicine , human cytomegalovirus , herpesviridae , viral disease , biology , virus , transplantation , biochemistry , surgery
Cytomegalovirus‐specific in vitro antibody production (CMV‐IVAP) by peripheral blood lymphocytes (PBL) was investigated in 12 renal transplant recipients. CMV‐IVAP, CMV‐serology, and viral cultures were carried out weekly during at least 8 weeks after transplantation. Nine episodes of CMV infection occurred in eight patients. CMV‐IVAP was positive in eight episodes; IgG and/or IgA and/or IgM antibodies reacting with several CMV polypeptides were secreted by PBL. In two patients with primary CMV infection, only IgA antibodies were detected. Cytomegalovirus cultures were positive in eight episodes and serological evidence of CMV infection was obtained in five episodes. In one case, CMV‐IVAP was observed before CMV isolation and in another case, before serological evidence of CMV infection. Our study indicates that CMV‐IVAP could represent an additional tool for the diagnosis of CMV infection. Our study indicates that CMV‐IVAP could represent an additional tool for the diagnosis of CMV infection in renal transplant recipients. © 1993 Wiley‐Liss, Inc.