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Comparative study of the isolation of human immunodeficiency virus from the lung and peripheral blood of aids patients
Author(s) -
Clarke John R.,
Williamson John D.,
Mitchell David M.
Publication year - 1993
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890390304
Subject(s) - bronchoalveolar lavage , virology , pneumocystis carinii , pneumonia , virus , lung , polymerase chain reaction , cytomegalovirus , medicine , immunology , viral disease , concomitant , biology , human immunodeficiency virus (hiv) , herpesviridae , pneumocystis jirovecii , biochemistry , gene
HIV-1 proviral DNA was detected by the polymerase chain reaction (PCR) in leucocytes from the peripheral blood (PBL) and bronchoalveolar lavage (BAL) of 100% and 72%, respectively, of HIV-1 seropositive patients. Infective virus isolated by cocultivation was recovered from the PBL and BAL of 50% and 59% of individuals, respectively. Isolation of HIV-1 was more readily made from the lung of individuals undergoing Pneumocystis carinii pneumonia (PCP) than from patients with non-PCP lung infections. The concomitant infection of lung cells with cytomegalovirus did not affect the isolation rate of HIV-1 from the lung. HIV-1 was isolated from BAL of 23 out of 36 (64%) individuals receiving no antiviral chemotherapy and from 13 out of 24 (54%) patients who were receiving AZT.