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Mechanism of astrovirus entry into Graham 293 cells
Author(s) -
Donelli Gianfranco,
Superti Fabiana,
Tinari Antonella,
Marziano Maria Luisa
Publication year - 1992
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890380408
Subject(s) - monensin , astrovirus , endocytosis , virology , cell culture , internalization , biology , immunofluorescence , rotavirus , virus , microbiology and biotechnology , cell , immunology , biochemistry , antibody , genetics
Astroviruses are intestinal pathogens associated with gastroenteritis in man and animals. The mechanism of internalization into host cells has not been reported previously. The cell entry pathway of serotype 1 human astrovirus into 293 cell line was studied biochemically and morphologically. Viral infection was monitored by indirect immunofluorescence. Infected cells were treated with the lysosomotropic agents ammonium chloride, methylamine, and dansylcadaverine or the ionophore monensin to raise the intraendosomal and intralysosomal pH. All drugs tested inhibited the early stages of infection whereas they did not interfere with the viral binding to the plasma membrane. The presence of astrovirus particles was detected by electron microscopy in coated pits and later in coated vesicles. The data indicate adsorptive endocytosis as the most probable mechanism by which astroviruses enter susceptible cells. © 1992 Wiley‐Liss, Inc.