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Serum IgA, IgG, and IgM responses to different enteroviruses as measured by a coxsackie B5‐based indirect ELISA
Author(s) -
Boman Jens,
Nilsson Birgitta,
Juto Per
Publication year - 1992
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890380108
Subject(s) - enterovirus , titer , virology , enterovirus 71 , serology , antibody , immunoglobulin m , antigen , immunology , coxsackievirus , medicine , biology , virus , immunoglobulin g , microbiology and biotechnology
An enterovirus‐specific indirect ELISA, based on a single local isolate of coxsackie B5 as antigen, was used to study the IgA, IgG, and IgM responses in 19 patients with a recent or current enterovirus infection. Twelve different enterovirus serotypes were isolated from 15 patients. Paired serum samples were available from 10 and a single serum from 5 of these 15 patients. In addition, 4 patients diagnosed by a significant titer rise of complement fixing antibodies to enterovirus were included. A serological diagnosis, defined as an increase in titer of enterovirus IgG and/or presence of enterovirus IgM, were established in all 14 patients with paired sera. Enterovirus IgM was present i n either a single serum or in both sera in 13 of them. Out of 5 patients with a single serum sample only, enterovirus IgA or enterovirus IgM was found in 4. Specific IgA was present in either a single serum or i n both sera in 14 of the 19 patients. Seven of the 10 enterovirus isolate patients with paired sera had a significant titer rise of complement fixing antibodies; however, all 10 were diagnosed by ELISA. Among 64 healthy controls 2 had enterovirus IgA and none had enterovirus IgM. In conclusion, the use of a single antigenbased ELISA was found t o be reliable for the diagnosis of recent and current enterovirus infections. © 1992 Wiley‐Liss, Inc.