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Characterisation by restriction mapping of three subtypes of Molluscum contagiosum virus
Author(s) -
Porter C. D.,
Archard L. C.
Publication year - 1992
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890380102
Subject(s) - restriction enzyme , bamhi , molluscum contagiosum , biology , virology , virus , genome , dna , restriction map , nucleic acid sequence , genetics , gene
DNA from Molluscum contagiosum virus (MCV) isolates was analysed by restriction endonuclease digestion, identifying three virus subtypes. The structural features of MCV DNA are typical of poxviral DNA. Physical maps of cleavage sites for BamHI, Clal, and Hindlll were constructed for single isolates of each subtype. These differ extensively, indicating the independence of the three subtypes. However, they are closely related, as determined by molecular hybridisation and nucleotide sequence analysis, and their genomes are essentially colinear. There is marked geographical variation in the relative incidence of MCV I and II, whilst MCV Ill is uniformly rare. © 1992 Wiley‐Liss, Inc.