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Improved serodiagnosis of chronic hepatitis C in Japan by a second‐generation enzyme‐linked immunosorbent assay
Author(s) -
Yuki Nobukazu,
Hayashi Norio,
Hagiwara Hideki,
Takehara Tetsuo,
Oshita Masahide,
Kasahara Akinori,
Fusamoto Hideyuki,
Kamada Takenobu
Publication year - 1992
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890370316
Subject(s) - virology , hepatitis c virus , recombinant dna , titer , medicine , virus , flaviviridae , polymerase chain reaction , chronic liver disease , reverse transcriptase , hepatitis c , reverse transcription polymerase chain reaction , serology , chronic hepatitis , hepatitis , antibody , biology , immunology , gene , gene expression , biochemistry , cirrhosis
To clarify the role of hepatitis C virus (HCV) infection in chronic liver disease, sera from Japanese patients which were negative by the original anti‐HCV assay (Ortho) were subjected to a second‐generation anti‐HCV assay based on a combination of structural (C22) and nonstructural (C200) recombinant HCV proteins. Of 29 patients with chronic non‐A, non‐B hepatitis, 20 (69%) were anti‐HCV‐positive by the second‐generation enzyme‐linked immunosorbent assay (ELISA) and also positive by the reverse transcription‐polymerase chain reaction (RT‐PCR) which detects the HCV genome. Of 41 chronic hepatitis B virus carriers, 3 (7%) were positive by the second‐generation ELISA; 1 of 3 was positive by RT‐PCR. The HCV genome was detected in ail cases positive for anti‐HCV with high titers. Of 59 healthy subjects negative by the second‐generation ELISA, none were positive by RT‐PCR. These findings indicate that HCV is a major causative agent of chronic non‐A, non‐B hepatitis in Japan and that second‐generation ELISA is specific and a more sensitive diagnostic assay for chronic hepatitis C. © 1992 Wiley‐Liss, Inc.