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Peripheral blood leukocytes of patients with argentine hemorrhagic fever as effectors of antibody‐dependent cell‐cytotoxicity
Author(s) -
Ambrosio Ana M.,
Gamboa Graciela S.,
Feuillade María R.,
Briggiler Ana M.,
Rimoldi M. T.
Publication year - 1992
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890370315
Subject(s) - antibody dependent cell mediated cytotoxicity , convalescence , peripheral blood mononuclear cell , effector , immunology , antibody , cytotoxicity , lytic cycle , microbiology and biotechnology , biology , medicine , in vitro , virus , biochemistry , monoclonal antibody
Peripheral blood mononuclear cells (PBMC) and polymorphonuclear leukocytes (PMNL) of patients with Argentine hemorrhagic fever (AHF) were tested as effectors (E) of antibody‐dependent cell cytotoxicity (ADCC). 51 Cr labeled chicken red blood cells (CRBC) coated with anti‐CRBC or normal rabbit serum were used as targets (T). Three ADCC assays were performed with both effectors from patients: on admission (I), 4 days after the transfusion of immune plasma (II), and 30 days after the clinical onset (III). The ADCC values obtained displayed high variation between individuals. From the linear portions in the curves representing specific 51 Cr release vs. E:T ratio plots, extrapolations were made t o determine lytic units (LU), defined here as effector concentrations required t o lyse 50% of the targets. The results were expressed as LU in 106 effector cells. The killing activity ranges of patients' PMNL (I = 1.04 ± 0.34; II = 2.22 ± 0.66; and III = 2.08 ± 1.18) were not significantly different from that of 21 normal controls (1.19 ± 0.361), except for range II ( P < 0.01 1). ADCC activity ranges of patients' PBMC (I = 3.40 ± 1.06; II = 3.16 ± 1.60; and III = 1.93 ± 0.42) were not significantly different from that determined in 12 healthy subjects (1.86 ± 0.40). These results demonstrate that patients' PBMC and PMNL can perform ADCC with efficiency comparable to normal effector cells, during the acute period of AHF, and in early convalescence. Consequently, ADCC can be a relevant mechanism in the clearance of Junin virus‐infected cells.