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High rate of multiple genital HPV infections detected by DNA hybridization
Author(s) -
LauricellaLefebvre M.A.,
Piette J.,
Lifrange E.,
Lambotte R.,
Gerard P.,
Rentier B.
Publication year - 1992
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890360406
Subject(s) - dot blot , southern blot , virology , biology , dna–dna hybridization , dna , nucleic acid thermodynamics , blot , sex organ , hybridization probe , microbiology and biotechnology , gene , base sequence , genetics
Cervical smears collected from 450 patients involved in a clinical follow‐up of cervical human papillomaviruses (HPV) infections were screened for the presence of HPV 6b, 11, 16, and 18 DNA by both dot blot and southern blot hybridization methods. Using very high stringency hybridization assays, the four HPV types could be easily distinguished by dot blotting. After a preliminary clinical sorting, 42.9% of the samples were found to be HPV‐positive. Among the samples infected by a single HPV, type 16 was the most frequent (25.4% of the positive samples) followed by 6b (19.7%), 11 (8.3%), and 18 (7.2%). Double or even multiple infections by the different HPV types were detected at a very high rate (39.4% of the positive samples).