Premium
Herpes simplex virus infection of cultured human term trophoblast
Author(s) -
NørskovLauritsen N.,
AboagyeMathisen G.,
Juhl C. B.,
Petersen P. M.,
Zachar V.,
Ebbesen P.
Publication year - 1992
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890360303
Subject(s) - virology , herpes simplex virus , trophoblast , biology , virus , term (time) , cell culture , medicine , microbiology and biotechnology , pregnancy , placenta , fetus , genetics , physics , quantum mechanics
Mononuclear trophoblast cells were isolated from term placentas of uncomplicated pregnancies, purified to homogeneity by negative immunomagnetic separation using monoclonal antibodies to the major histocompatibility complex, and challenged with herpes simplex virus (HSV). The cultures were highly susceptible to virusinduced cytopathic effect as evidenced by cytopathic alteration and inhibition of human chorionic gonadotropin (hCG) secretion. Both HSV I and II underwent a full replicative cycle in the trophoblast, although the production of progeny virus was 10‐100 times less than that obtained with placental fibroblasts or choriocarcinoma cells. The permissiveness was independent of in vitro syncytial differentiation of the trophoblast. The results suggest that the trophoblast layer may be involved in intrauterine HSV infection.