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Low risk of transmission of the human immunodeficiency virus by a solvent‐detergent‐treated commercial factor VIII concentrate
Author(s) -
Paolantonio Tiziana D.,
Mariani Guglielmo,
Ghirardini Alessandro,
Gringeri Alessandro,
Mannucci Pier Mannuccio,
Mastrullo Lucia,
de Biasi Raffaello,
Giustarini Gloria,
Morfini Massimo,
Schiavoni Mario,
Ciavarella Nicola,
Zehender Gianguglielmo,
Zanetti Alessandro Remo
Publication year - 1992
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890360202
Subject(s) - virology , antibody , serology , human immunodeficiency virus (hiv) , antigen , virus , viral disease , medicine , polymerase chain reaction , western blot , immunology , biology , biochemistry , gene
A study evaluating the risk of a commercial factor VIII (FVIII) concentrate's transmitting the human immunodeficiency virus (HIV) was carried out on hemophiliacs, by using multiple serological markers and the polymerase chain reaction (PCR). Twenty‐nine hemophiliacs, negative for HIV antibodies, were treated for 18 months with a concentrate that had been inactivated by solvent‐detergent. HIV‐1 antibodies and antigen were assayed during the follow‐up period. At the end of the study, all patients were also tested by the HIV 1+2 combined antibody assay; Western blot (WB) antibody analysis; and in eight cases, by an HIV‐1 PCR technique. Patients received a yearly median FVIII dose of 35,330 IU (range 3,300‐306,000); the median number of lots given to each patient was 6 (1‐45). During the follow‐up period and at the end of the study, HIV‐1 antibodies and antigen were not detected in any of the subjects. The HIV 1+2 combined assay and WB analysis carried out only at the end of the study were negative. HIV‐1 PCR was negative in all the tested patients. This study has shown that this solvent‐detergent‐treated FVIII concentrate did not transmit HIV.