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Direct serotyping of porcine rotaviruses using VP7‐specific monoclonal antibodies by an enzyme immunoassay
Author(s) -
Nagesha H. S.,
Holmes I. H.
Publication year - 1991
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890350311
Subject(s) - serotype , polyclonal antibodies , virology , monoclonal antibody , antiserum , immunoassay , biology , antigen , rotavirus , antibody , virus , immunology
Employing a serotyping EIA test using Mabs both cell culture adapted and faecal porcine rotaviruses were classified into serotypes G3, G3/5, G4, and G5. The MAbs have confirmed and extended the serotyping results obtained using polyclonal antisera. These MAbs are therefore potential reagents for serotyping of porcine ro‐taviruses. Using subgroup specific MAbs sero‐types G3, G3/5, and G5 were found to contain subgroup I antigens while G4 rotaviruses contained either subgroup II or subgroup I antigens.