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Persistence of cytomegalovirus in human long‐term bone marrow culture: Relationship to hemopoiesis
Author(s) -
Preiksaitis Jutta K.,
JanowskaWieczorek Anna
Publication year - 1991
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.1890350203
Subject(s) - virology , biology , betaherpesvirinae , haematopoiesis , bone marrow , cytomegalovirus , persistence (discontinuity) , titer , human cytomegalovirus , population , progenitor cell , immunology , stromal cell , peripheral blood mononuclear cell , macrophage , herpesviridae , in vitro , virus , viral disease , stem cell , medicine , biochemistry , genetics , geotechnical engineering , environmental health , cancer research , engineering
Using pre‐established human long‐term marrow culture (LTMC), we studied cytomegalovirus (CMV) replication in this system after in vitro infection of nonadherent cells obtained from these cultures with CMV AD‐169. After infection with 5 immediate‐early antigen foci/cell CMV was detectable for 63–123 days (peak titer 2.0 × 10 3 −1.3 × 10 9 ) in the supernatants of LTMC. Lower MOI resulted in a delay in the detection and longer persistence of CMV in LTMC although peak titers were unchanged. CMV infection was associated with destruction of the stromal layer, appearance of a subset of large (23 μ) CMV‐infected mononuclear cells in the nonadherent fraction, and early differentiation of nonadherent cells into a homogenous population of macrophage‐like cells. CMV infection resulted in a reduction and premature disappearance of committed progenitors (BFU‐E, CFU‐GM) in LTMC. Persistence of CMV in LTMC was linked to ongoing hemopoiesis. Human bone marrow may be an important site for CMV replication during acute infection and CMV persistence.